Prague Fertility Centre: Infertility treatment in Czech Republic, Prague
Specialist infertility treatment in Prague, Czech
Republic offering IVF: in-vitro fertilisation, ICSI: intracytoplasmic
sperm injection, PICSI technique, permanent embryo monitoring, PGD/PGS,
extended embryo culture, assisted hatching, embryo freezing, sperm
freezing, egg donation, sperm donation, and embryo donation.
News: Prague fertility centre offers package of 3 full IVF cycles for the price of 2.
Results worldwide show without doubt that the 'cumulative‘ success rate
is always higher than that from one cycle. Their aim of keeping costs down is to
encourage the idea of IVF as a multiple cycle treatment. Success is more likely
to be achieved if couples accept IVF as a course of treatment rather than a one
Prague Fertility Centre (PFC) is a world-class fertility centre in
Czech Republic. PFC is co-led by the widely respected embryologist Dr.
Daniel Hlinka PhD., who was the first to introduce permanent monitoring
of embryo development, which is why PFC is the only fertility centre in the Czech Republic (and amongst the very few in the world) that performs this new and effective method in infertility treatments.
Your treatment in their centre is absolutely transparent - you receive a
video report of your embryo development recorded by newly introduced
permanent embryo monitoring. Specialists at PFC take care of you in a
friendly and relaxed way. They put a great emphasis on the psychological
aspect of your treatment and provide you with faith and certainty.
Prague Fertility Centre guarantee system
In one egg/embryo donation cycle PFC always guarantee:
In their guarantee system PFC would like to minimize
the biologically based variations in the process of donor stimulations.
Accordingly, there are several situations which are calculated and
solved as follows:
if there is no one embryo for embryo transfer: - they
prepare a new egg donor (this is a very rare case but they cannot exclude
such a situation);
there are 4 and more good quality embryos: 2-3
embryos are transferred (according to the age and history of every
woman) and the rest ones are vitrified for future use;
there are just 2 or 3 good quality embryos: - these
embryos are used for ET. If no pregnancy is established after ET (or
when after successful pregnancy and delivery of the baby a new attempt
is planed) they prepare a new egg donor to produce embryos for guaranteed
ET of cryopreserved (vitrified) embryos.
Moreover, in their egg/embryo donation they assure
your pregnancy in the two following donation cycles - otherwise the
third cycle is free (provided the normal i.e. at least 50% fertilization
rate was obtained using your partner's spermatozoa - in egg
Please see more at http://www.pragueivf.com/en/donation-program/guaranties/.
The Prague Fertility Centre approach to infertility treatment
To provide comprehensive, innovative, evidence-based fertility and gynaecology treatment focused on individual problems and needs.
1. PICSI for the best spermatozoa selection
2. Monitoring of embryo development:
a. to select the best embryo and to avoid early miscarriages
b. to make infertility treatment absolutely transparent (following the
progress of embryo development on-line and exact analysis of developmental processes)
Prague Fertility Centre specialises in infertility treatment for people over 40
Prague Fertility Centre
is the first accredited centre working with human tissue in the Czech
Republic compliant with the Directive of the European Parliament and
Council 2004/23/ES and Law no. 296/2008 from the collection of laws and
legal directives no. 422/2008. It has a permit issued by the National
Institute for Medication Control (SUKL).
There is no waiting list for egg donation with Prague Fertility Centre
Sonja Lazarovska MD.
Dr Sonja Lazarovska MD. is a gynecologist-obstetrician with Prague Fertility Centre: specialised in reproductive medicine.
Sonja has more than 20 years of practice in the field with specialisation in gynecology and obstetrics:
13 years with the Institute for Obstetric and
Pediatric Care, Podoli, Prague, where she was mostly dedicated to
prenatal diagnostics including invasive diagnostic intervention
Over 8 years with the Sanatorium Pronatal, Prague in the field of reproductive medicine
During this time she has been involved in complex
diagnostics, sterility treatment with all methods of assisted
reproduction available, endoscopic surgery, and reproductive
Daniel Hlinka MVD., PhD.
Dr Daniel Hlinka MVD., PhD. ESHRE certified Senior Clinical Embryologist: one of the most experienced Czech and Slovak embryologists:
He was the first to apply the ICSI method
successfully in the Slovak Republic, resulting in the birth of the first
such child in 1994.
He contributed substantially to the introduction
of preimplantation genetic diagnosis/screening (PGD/PGS) in the
Previously he has led embryological laboratories in:
II. gyn.-obst. clinic Košice – Slovakia;
KRM Zlín, Repromeda Brno, Pronatal Prague – Czech rep.
PFC offers the newest ART treatments for successful infertility treatment
Production of healthy and viable embryos is a crucial step in achieving successful infertility treatment.
Prague Fertility Centre (PFC) provides highly personalised care, support, and treatment options. They aim to do everything they can to maximise your chances of having a healthy baby.
PFC has highly qualified and experienced staff which will help you through your treatment.
Their efforts are supported through PFC research programs and their association with other research programs.
The Prague Fertility Centre specialises in infertility treatment for people over 40.
There is no waiting list for egg donation at PFC in Czech Republic
Prague Fertility Centre only uses eggs from healthy young donors and they never use surplus eggs from their patients. The donors
undergo hormone screening, an ultrasound examination, psychological
screening, and repeated tests for sexually transmitted diseases (HIV,
HBsAg, HCV, syphilis). They also undergo karyotype screening and genetic
screening to reveal the presence of CFTR gene mutations which cause
cystic fibrosis. Their donors are usually university students or young mothers on maternity leave. None of them are treated for infertility and egg donation is their free choice. Czech law states that egg donation
is anonymous: It is not possible for the donor to know the identity of
the recipient or vice versa. It is however possible to choose a donor
with certain specific characteristics – height, weight, hair and eye
color. It is also possible to choose a donor with the same blood group like the recipient.
If you are interested in egg donation or require further information about donation, please don’t hesitate to contact Prague Fertility Centre.
Sperm donation at Prague Fertility Centre
involves using semen from an appropriately screened man (donor). The
semen is used to fertilise eggs during assisted reproductive treatment.
In their embryo donation program Prague Fertility Centre can offer just embryos that have been created using donated eggs and donated spermatozoa.
Prague Fertility Centre
do not use already existing cryopreserved surplus embryos that have
been created using IVF techniques and are in excess to the needs of the
couple who created them.
If you are interested in embryo donation or require further information about donation, please don’t hesitate to contact Prague Fertility Centre.
IVF: in-vitro fertilisation
eggs are placed together with washed spermatozoa and fertilisation
occurs spontaneously, as in the oviducts of the mother. A disadvantage
of this method is the low fertilisation rate, mainly in cases when the
partner’s sperm is low-quality. Often fertilisation
is unsuccessful even though sperm is assessed as good quality in semen
analysis. Sometimes polyspermy occurs, when more than one sperm
fertilise an egg; the resulting embryos are abnormal (have more
chromosomes than normal) and so are rejected.
ICSI: intracytoplasmic sperm injection
one sperm is injected directly into the egg cytoplasm using an
apparatus that transforms imperfect hand movements into fine and precise
movements of micromanipulation tools. This method is used in the following cases:
Abnormal semen analysis findings. I.e. low sperm concentration or a high level of abnormal sperm with low motility
Fertilisation using sperm surgically extracted from testicles or the epididymis (using MESA/TESE techniques)
Failure to spontaneously fertilise in the previous cycle even though semen analysis results are positive
PICSI technique improves pregnancy rates and reduces the number of IVF miscarriages
In the ICSI
procedure, an individual sperm is selected and injected into an oocyte.
Until now, the only technique available to embryologists to select the
sperm has been visual observation. Using the PICSI procedure Prague Fertility Centre is able to determine sperm selection in much the same way it happens in human biology.
Sperms are placed in a PICSI dish
containing samples of hyaluronan hydrogel. Hyaluronan is a naturally
occurring biopolymer found in all human cells, including the gel layer
surrounding the oocyte.
Mature, biochemically competent sperm bind to the hyaluronan where they can be isolated by the embryologist and used for ICSI.
This procedure mimics a key step in the natural fertilisation process;
the binding of mature sperm to the oocyte complex. As a result, the
selected sperm is essentially the same as one that would be successful
in the natural reproductive process.
Research has proved that hyaluronan-bound PICSI-selected
sperm are, in the vast majority of cases, more mature, exhibit less DNA
damage, and have fewer chromosomal aneuploidies.
Click here to view a video of PICSI mature spermatozoa selection.
Click here to view a video of PICSI human egg fertilisation by selected spermatozoon.
Non-invasive embryo imaging with detection of abnormal divisions leading to aneuploidy
Objective and non-invasive imaging of embryo
characteristics allows detection of abnormal cleavages leading to the
occurence of chromosomal malsegregation which are a major cause of
spontaneous miscarriage or birth defects PFC offers a noninvasive
aneuploidy prediction as an alternative to invasive genetic aneuploidy
screening (requires biopsy procedure for 1-2 embryonic cells removal).
Non-invasive aleuploidy prediction examples
Monitoring of early embryonic cleavages allows reliable aneuploidy prediction
The evalutaion of embryonic developmental abilities
according to the static microscopic observation is negatively influenced
by a great subjectivity in the assessment and by questionable relevance
of the measured morphological parameters. On the other side the new
imaging technologies allow continous embryo monitoring and subsequent
picture analysis. Using non-invasive embryo imaging Prague Fertility Centre can reveal mosaic embryos consisting of aneupliod/euploid cells frequently leading to early abortions
Click here to view more about embryo monitoring.
PGD/PGS: reimplantation genetic diagnosis/ preimplantation genetic screening
Genetic evaluation of embryos before transfer: the embryo's development does not always reflect its genetic constitution.
After in-vitro fertilisation was introduced into clinical practice, preimplantation genetic diagnosis (PGD) was put forward as an alternative to prenatal diagnosis of genetically transmitted diseases.
In the first place it was supposed to solve the dilemma
as to whether a pregnancy should be terminated if the embryo was found
to be carrying a genetic defect.
Gradually genetic screening of embryos became used more
and more in practice to detect de novo emerging anomalies (chromosomal
anomalies emerging in the course of gametogenesis and during early
embryo development); preimplantation genetic screening (PGS).
Click here to view a video of PGD/PGS biopsy of a human embryo.
Click here to view a video of PGD/PGS aneuploid embryo detection using permanent monitoring.
Extended embryo culture
Extended embryo culture
is a term used to describe the embryo remaining in a culture medium
from the third day after fertilisation. Its advantage is that it allows
one to judge the embryo’s development and morphological characteristics.
Permanent monitoring of the embryo’s development provides Prague Fertilty Centre with
much more information about the dynamics of cell division and very
often the healthiest embryos suitable for transfer are already known at
day 2 of culturing.
Culture duration is also influenced by the following factors:
1) Endometrial development (growth of uterine lining)
2) Possible occurrence of hyperstimulation syndrome
3) Time necessary for genetic screening of biopsy cells during PGD/PGS
Assisted hatching (AH)
Disruption of the outer embryo layer: this helps
implantation of the embryo in the uterus. On the 5th or 6th day of its
development, the embryo leaves its protective outer layer (zona
pellucida), which among other things has protected it till now from
extracorporeal manipulation. This process of breaking out or “hatching”
is necessary for the embryo to establish contact with uterus cells
become implanted. Some embryos are not able to undergo this process and
they remain lodged in the zona pellucida. This is why scientists have
come up with the assisted hatching (AH)
technique. Using this technique the zona pellucida is breached in one
place using micromanipulation tools which helps the embryo hatch out of
its layer. Although today there are many varying opinions about AH,
it is generally believed that healthy embryos are not affected by this
technique. Patients should not overrate the effectiveness of AH.
Click here to view a video of assisted hatching.
Freezing surplus embryos
Preserving embryos for use at a later time: there are many reasons why a patient may want to freeze embryos when undergoing infertility treatment, the most common ones are:
Risk of hyperstimulation syndrome
Inadequate growth of uterine lining
Medical finding on the reproductive organs which prevents the creation of conditions necessary for embryo implantation
Other acute diseases that are incompatible with pregnancy
Freezing of the spermatozoa in advance
In the egg donation program it is considered appropriate to freeze sperm before oocytes are retrieved.
Contact details for infertility clinic in Czech Republic
Prague Fertility Centre
Mirna Turcinovic, Program Coordinator: firstname.lastname@example.org
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